In the first inning of Cleveland's 8-2 win against the Twins, Crowe dove for a fly ball. As he extended his left arm forward to make the catch, the ball caromed off his mitt. Kosuke Fukudome replaced Crowe after the inning.
"He was uncomfortable out there, and as soon as he came in, he was checked out by our medical staff," Tribe manager Manny Acta said. "He's getting some tests done right now, but he's gone, like a chicken through the corn."
Crowe joins Michael Brantley and Shin-Soo Choo as Indians outfielders who will not play again this season.
"That's why I stopped working out the last week," Acta said, "because I want to make it through the season. I'm the only one left."
Crowe was limited to just 15 games this season after he had surgery on his right shoulder on March 30.
The Indians transferred Choo to the 60-day disabled list on Saturday. Choo, out with a strained left oblique, first landed on the 15-day DL after suffering the injury during a swing on Aug. 27. He returned to the lineup on Sept. 15, but aggravated the strain during his first at-bat.
The roster move allowed the Indians to recall right-handed pitcher Mitch Talbot from Triple-A Columbus. Talbot (2-6, 6.33) was to start the nightcap of Saturday's twin bill.
Zack Meisel is an associate reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.